Mouse Collagen alpha-1(XVIII) chain (COL18A1/ES) ELISA kit

大包装询价

货号: IPD-E07974m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠胶原蛋白α-1(XVIII)链(COL18A1/ES)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的COL18A1/ES含量。COL18A1是XVIII型胶原蛋白的α链，参与细胞外基质构建，具有抗血管生成作用，与视网膜结构和神经管闭合相关。研究显示，COL18A1通过调节细胞迁移、增殖和凋亡等过程影响血管生成和肿瘤生长。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外研究小鼠模型中COL18A1的表达调控机制，如血管生成相关疾病研究、肿瘤微环境分析或组织修复过程探索，尤其适用于比较不同处理条件下（如基因敲除、药物干预）生物样本中目标蛋白的动态变化，为分子机制研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Col18a1Collagen alpha-1(XVIII) chain [Cleaved into: Endostatin; Non-collagenous domain 1 ELISA Kit; NC1)] ELISA Kit
缩写：
COL18A1/ES
Uniprot No.：
P39061
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, cell culture supernates, tissue homogenates
检测范围：
0.156 ng/mL-10 ng/mL
灵敏度：
0.039 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse COL18A1/ES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-95
1:2	Average %	103
	Range %	99-105
1:4	Average %	92
	Range %	87-96
1:8	Average %	99
	Range %	94-101

回收率：

The recovery of mouse COL18A1/ES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-98
EDTA plasma (n=4)	95	91-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














ng/ml	OD1	OD2	Average	Corrected
10	2.234	2.213	2.224	2.067
5	1.732	1.694	1.713	1.556
2.5	1.091	1.076	1.084	0.927
1.25	0.551	0.537	0.544	0.387
0.625	0.366	0.386	0.376	0.219
0.312	0.292	0.281	0.287	0.130
0.156	0.248	0.228	0.238	0.081
0	0.159	0.155	0.157

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.