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Human Collagen alpha-1(XVII) chain (COL17A1) ELISA kit

  • 中文名： 人XVII型胶原α-1链(COL17A1)酶联免疫试剂盒
货号: IPD-EL005724HU
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人XVII型胶原α-1链(COL17A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的COL17A1含量。COL17A1是一个靶点。它与皮肤等组织健康相关。研究机制上，围绕其在细胞增殖、分化及组织稳态维持等方面展开，探究其表达调控、信号传导通路等，揭示其在生理和病理过程中的作用，为相关疾病防治提供方向。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于皮肤生物学、细胞外基质研究、创伤愈合机制探索等科研领域，为体外研究COL17A1在组织稳态维持或病理状态中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    COL17A1 ELISA kit; BP180 ELISA kit; BPAG2Collagen alpha-1(XVII) chain ELISA kit; 180 kDa bullous pemphigoid antigen 2 ELISA kit; Bullous pemphigoid antigen 2) [Cleaved into: 120 kDa linear IgA disease antigen ELISA kit; 120 kDa linear IgA dermatosis antigen ELISA kit; Linear IgA disease antigen 1 ELISA kit; LAD-1); 97 kDa linear IgA disease antigen ELISA kit; 97 kDa linear IgA bullous dermatosis antigen ELISA kit; 97 kDa LAD antigen ELISA kit; 97-LAD ELISA kit; Linear IgA bullous disease antigen of 97 kDa ELISA kit; LABD97)] ELISA kit
  • 缩写：
    COL17A1
  • Uniprot No.：
    Q9UMD9
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human COL17A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    103


    Range %

    96-110


    1:2

    Average %

    94


    Range %

    89-100


    1:4

    Average %

    97


    Range %

    92-103


    1:8

    Average %

    98


    Range %

    91-104


  • 回收率：

    The recovery of human COL17A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    99

    92-106


    EDTA plasma (n=4)

    94

    88-100


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    10

    2.852

    2.934

    2.893

    2.722


    5

    2.023

    2.097

    2.060

    1.889


    2.5

    1.504

    1.528

    1.516

    1.345


    1.25

    0.988

    0.999

    0.993

    0.822


    0.625

    0.552

    0.521

    0.536

    0.365


    0.312

    0.438

    0.453

    0.446

    0.275


    0.156

    0.287

    0.284

    0.285

    0.114


    0

    0.167

    0.175

    0.171




  • 货期：
    3-5 working days

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