首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人XI型胶原α-1链(COL11A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的COL11A1含量。COL11A1 是重要靶点。它是编码胶原蛋白 XIα1 链的基因。在人体组织发育和稳态维持中起作用。相关研究聚焦其在肿瘤微环境重塑等机制，发现它与肿瘤进展、转移和预后等相关，有望成为肿瘤治疗的潜在靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于科研领域研究COL11A1在组织工程、疾病模型中的表达调控，或探索其在细胞外基质重塑、胚胎发育及遗传性疾病中的分子机制本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：COBA1_HUMAN ELISA Kit; COL11A1 ELISA Kit; COLL6 ELISA Kit; Collagen alpha 1 ELISA Kit; Collagen alpha-1(XI) chain ELISA Kit; collagen XI alpha 1 ELISA Kit; collagen XI; alpha 1 polypeptide ELISA Kit; collagen; type XI; alpha 1 ELISA Kit; STL2 ELISA Kit; STL3 ELISA Kit; XI chain precursor ELISA Kit
缩写：COL11A1
Uniprot No.：P12107
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human COL11A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 98-107 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 91-98 | |||||
回收率：
| The recovery of human COL11A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-96 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.5022.4562.4792.30920002.0642.0862.0751.90510001.6811.6451.6631.4935001.0841.0461.0650.8952500.6810.6640.6730.5031250.4930.4630.4780.30862.50.2810.2690.2750.10500.1720.1680.170 | ||||||
货期：3-5 working days
×
