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产品描述：人X型胶原α-1链(COL10A1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的COL10A1含量。人X型胶原α-1链（COL10A1）是一种胶原蛋白，主要研究其在骨关节疾病中的作用。近年来，COL10A1在肿瘤中的作用受到关注，其可能通过改变肿瘤微环境成分，促进肿瘤发生和转移，具有成为多肿瘤治疗靶点的潜力。研究机制涉及COL10A1与肿瘤免疫微环境的关系，以及其在肿瘤细胞增殖、迁移和侵袭中的作用。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于科研领域探索骨骼生长、软骨分化、骨关节疾病模型中的分子机制，或用于体外培养细胞中COL10A1表达水平的定量分析，为骨代谢相关基础研究及药物开发提供可靠的数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：COAA1_HUMAN ELISA Kit; Col10a 1 ELISA Kit; COL10A1 ELISA Kit; Collagen alpha 1(X) chain ELISA Kit; Collagen alpha-1(X) chain ELISA Kit; Collagen type X alpha 1 (Schmid metaphyseal chondrodysplasia) ELISA Kit; Collagen type X alpha 1 ELISA Kit; Collagen X alpha 1 polypeptide ELISA Kit; CollagenX ELISA Kit; fa66d11 ELISA Kit; fb10c08 ELISA Kit; OTTHUMP00000040411 ELISA Kit; Procollagen type X alpha 1 ELISA Kit; Schmid metaphyseal chondrodysplasia ELISA Kit; wu:fa66d11 ELISA Kit; wu:fb10c08 ELISA Kit
缩写：COL10A1
Uniprot No.：Q03692
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human COL10A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-106 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-101 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human COL10A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-101 | ||||
| EDTA plasma (n=4) | 92 | 87-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected203.0393.0523.0462.909102.6472.6582.6532.51651.8982.0401.9691.8322.51.2111.2621.2371.1001.250.7080.6630.6860.5490.6250.4250.4060.4160.2790.3120.2420.2570.2500.11300.1380.1360.137 | ||||||
货期：3-5 working days
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