首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠Ⅳ型胶原(Col Ⅳ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Col Ⅳ含量。Col Ⅳ即Ⅳ型胶原，是基底膜的主要成分。它在维持组织正常结构与功能中发挥重要作用。研究发现其异常表达与肿瘤侵袭、转移及多种疾病相关，机制可能涉及与细胞表面受体相互作用，影响细胞黏附、迁移和信号传导等过程。试剂盒检测范围为1.56 ng/mL-100 ng/mL，可应用于肝/肾纤维化动物模型构建、血管基底膜损伤研究、肿瘤微环境分析等科研领域，特别适用于评估疾病模型中Col Ⅳ的动态变化及组织损伤修复机制，为心血管疾病、器官纤维化等病理过程的分子机制研究提供精准检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Collagen Type 4,Col 4,_x000D_ Collagen Type IV,Col IV
缩写：Col Ⅳ
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat COL Ⅳ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-94 | |||||
回收率：
| The recovery of rat COL Ⅳ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 104 | 99-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 1002.2212.1202.1712.019 501.4411.3401.3911.239 250.8250.8040.8150.663 12.50.5190.5300.5250.373 6.250.3350.3240.3300.178 3.120.2400.2510.2460.094 1.560.1960.1920.1940.042 00.1520.1510.152 | ||||||
货期：3-5 working days
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