首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人睫状神经营养因子(CNTF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CNTF含量。CNTF（睫状神经营养因子）是一种由睫状神经营养因子基因编码的蛋白质，属于IL-6细胞因子家族。它具有神经营养和分化作用，能促进神经递质的合成和某些神经元群的轴突生长。CNTF在神经保护和视网膜神经节细胞存活中发挥重要作用，是青光眼等神经退行性疾病研究的热点靶点。试剂盒检测范围为6.25 pg/mL-400 pg/mL，本产品适用于体外实验研究CNTF在神经系统、代谢调节或炎症反应中的作用机制，可应用于神经生物学、细胞信号通路研究以及疾病动物模型中神经营养因子水平的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ciliary neurotrophic factor ELISA Kit; CNTF ELISA Kit; CNTF_HUMAN ELISA Kit; HCNTF ELISA Kit
缩写：CNTF
Uniprot No.：P26441
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CNTF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 83-91 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 95-103 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 83-89 | |||||
回收率：
| The recovery of human CNTF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-103 | ||||
| EDTA plasma (n=4) | 90 | 85-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 4002.5042.5982.5512.369 2002.1942.0532.1241.942 1001.5551.5011.5281.346 500.8770.8980.8880.706 250.4510.4650.4580.276 12.50.3750.3620.3690.187 6.250.2740.2980.2860.104 00.1810.1830.182 | ||||||
货期：3-5 working days
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