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产品描述：人调宁蛋白- 1(CNN1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的CNN1含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本产品适用于基础医学研究、药效评估及疾病机制探索等科研场景，为心血管病理研究、抗纤维化药物开发及肿瘤微环境分析提供可靠检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Basic calponin ELISA Kit; Calponin 1 ELISA Kit; Calponin 1 basic smooth muscle ELISA Kit; Calponin H1 ELISA Kit; Calponin H1 smooth muscle ELISA Kit; Calponin-1 ELISA Kit; Calponin1 ELISA Kit; Calponins basic ELISA Kit; CNN 1 ELISA Kit; Cnn1 ELISA Kit; CNN1_HUMAN ELISA Kit; Epididymis secretory protein Li 14 ELISA Kit; HEL S 14 ELISA Kit; Sm Calp ELISA Kit; SMCC ELISA Kit; smooth muscle ELISA Kit
缩写：CNN1
Uniprot No.：P51911
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.156 ng/ml-10 ng/ml
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human CNN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 84-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 85-99 | |||||
| 1:8 | Average % | 104 | ||||
| Range % | 98-108 | |||||
回收率：
| The recovery of Human CNN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-98 | ||||
| EDTA plasma (n=4) | 87 | 83-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.2552.3452.3002.202 51.7151.7561.7361.638 2.51.0641.0241.0440.946 1.250.5230.5150.5190.421 0.6250.3340.3240.3290.231 0.3120.2510.2340.2430.145 0.1560.1620.1590.1610.063 00.0970.0980.098 | ||||||
货期：3-5 working days
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