首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人钙调蛋白2(CNN2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CNN2含量。人钙调蛋白2（CNN2）是一种在细胞信号传导中起关键作用的蛋白质，其研究背景涉及其在细胞内信号传递中的角色。CNN2的机制主要涉及调节钙离子浓度，影响平滑肌收缩，与心血管疾病和神经生物学相关。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于体外培养细胞模型、动物组织样本或体液样本的蛋白表达分析，为探索 CNN2 在细胞应激响应、组织再生机制或疾病相关分子通路中的功能提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Calponin 2 ELISA Kit; Calponin H2 ELISA Kit; Calponin H2, smooth muscle ELISA Kit; Calponin-2 ELISA Kit; Cnn2 ELISA Kit; CNN2_HUMAN ELISA Kit; Neutral calponin ELISA Kit; smooth muscle ELISA Kit
缩写：CNN2
Uniprot No.：Q99439
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CNN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of human CNN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 98-106 | ||||
| EDTA plasma (n=4) | 94 | 86-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.3252.4132.3692.254 10001.8761.9411.9091.794 5001.3741.2821.3281.213 2500.7520.7640.7580.643 1250.4340.4410.4380.323 62.50.2820.2970.2900.175 31.250.1830.1870.1850.070 00.1140.1150.115 | ||||||
货期：3-5 working days
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