Human Carboxymethyl lysine,CML ELISA Kit

大包装询价

货号: IPD-E12798h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人羧甲基赖氨酸(CML)酶联免疫试剂盒，双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CML含量。CML即慢性髓系白血病，是一种起源于多能造血干细胞的血液系统恶性克隆性疾病，与费城染色体及BCR-ABL融合基因相关。研究机制聚焦于该基因异常导致的酪氨酸激酶持续活化，目前多通过靶向抑制该激酶来开展治疗。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于基础科研中AGEs代谢机制探索、抗衰老药物功效评估以及糖尿病相关动物模型或细胞实验的分子标记物检测。其宽泛的样本兼容性支持跨组织类型的比较研究，为营养干预、代谢性疾病机理及氧化损伤相关领域提供可靠工具。助力研究者深入解析CML在慢性疾病发生发展中的生物学作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：
CML
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
62.5 pg/mL-4000 pg/mL
灵敏度：
15.6 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CML in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-95
1:2	Average %	94
	Range %	90-100
1:4	Average %	95
	Range %	91-105
1:8	Average %	94
	Range %	85-100

回收率：

The recovery of human CML spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-98
EDTA plasma (n=4)	94	91-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














pg/ml	OD1	OD2	Average	Corrected
4000	2.372	2.252	2.312	2.162
2000	1.775	1.660	1.718	1.568
1000	1.160	1.142	1.151	1.001
500	0.712	0.699	0.706	0.556
250	0.493	0.456	0.475	0.325
125	0.342	0.329	0.336	0.186
62.5	0.264	0.249	0.257	0.107
0	0.153	0.147	0.150

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.