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产品描述：人封闭蛋白4(CLDN4)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CLDN4含量。CLDN4是一个关键靶点。它属于紧密连接蛋白家族，在多种上皮和内皮组织中表达。其表达异常与肿瘤发生、发展及转移密切相关。研究机制主要聚焦于其在肿瘤细胞增殖、迁移等过程中的作用机制，以及探索以此为靶点的治疗策略。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于体外培养细胞、动物模型组织或体液样本中CLDN4的检测，为肿瘤微环境研究、上皮屏障功能探索及炎症相关分子机制等科研领域提供可靠工具，满足基础研究中对蛋白表达差异分析的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CLDN4; CPER; CPETR1; WBSCR8; Claudin-4; Clostridium perfringens enterotoxin receptor; CPE-R; CPE-receptor; Williams-Beuren syndrome chromosomal region 8 protein
缩写：CLDN4
Uniprot No.：O14493
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CLDN4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 89-97 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 94-103 | |||||
回收率：
| The recovery of human CLDN4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 86-97 | ||||
| EDTA plasma (n=4) | 85 | 80-90 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected4002.3342.2272.2812.1652001.8581.7521.8051.6891001.3891.3681.3791.263500.9720.9840.9780.862250.6980.6820.6900.57412.50.4960.4850.4910.3756.250.2910.2840.2880.17200.1170.1150.116 | ||||||
货期：3-5 working days
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