首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人封闭蛋白3(CLDN3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CLDN3含量。CLDN3是一个重要靶点。它属于紧密连接蛋白家族成员，在维持细胞极性和屏障功能等方面有重要作用。研究显示其异常表达与多种肿瘤发生、发展相关，可能作为潜在治疗靶点，目前相关研究聚焦于其作用机制及开发针对性治疗策略。试剂盒检测范围为25 pg/mL-1600 pg/mL，广泛应用于肿瘤生物学、上皮屏障功能调控、药物作用机制等研究领域，例如探索肿瘤转移过程中CLDN3的动态表达、评估炎症或药物干预对上皮细胞连接完整性的影响等，为体外实验提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CLDN3; C7orf1; CPETR2; Claudin-3; Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-receptor 2; Rat ventral prostate.1 protein homolog; hRVP1
缩写：CLDN3
Uniprot No.：O15551
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CLDN3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 86-93 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 85-92 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 98-105 | |||||
回收率：
| The recovery of human CLDN3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 93-102 | ||||
| EDTA plasma (n=4) | 87 | 80-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected16002.1162.2942.2052.0098001.6351.5321.5841.3884001.1441.1241.1340.9382000.7730.7500.7620.5661000.5420.5370.5400.344500.4230.4390.4310.235250.3020.3090.3060.11000.1950.1960.196 | ||||||
货期：3-5 working days
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