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产品描述：人封闭蛋白2(CLDN2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CLDN2含量。CLDN2 即闭合蛋白 2，是紧密连接蛋白家族成员。它在维持细胞极性及屏障功能等方面有重要作用。研究发现其在多种肿瘤中异常表达，可能参与肿瘤细胞增殖、迁移等过程，有望成为肿瘤诊断和治疗的新靶点。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，该产品广泛应用于细胞生物学、病理机制及药物干预等基础研究，例如探究上皮屏障功能障碍模型、炎症性疾病动物实验或肿瘤细胞间连接相关分子机制，为科研人员提供可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CLDN2; PSEC0059; SP82; UNQ705/PRO1356; Claudin-2; SP82
缩写：CLDN2
Uniprot No.：P57739
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CLDN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 88-98 | |||||
回收率：
| The recovery of human CLDN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 100-109 | ||||
| EDTA plasma (n=4) | 96 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.6712.5522.6122.4497502.1242.0062.0651.9023751.4381.4031.4211.258187.50.8060.8390.8230.660940.5080.5240.5160.353470.2940.3090.3020.13923.50.2340.2270.2310.06800.1610.1640.163 | ||||||
货期：3-5 working days
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