| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARL4D |
| Uniprot No | P49703 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-201aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGNHLTEMAPTASSFLPHFQALHVVVIGLD SAGKTSLLYRLKFKEFVQ SVPTKGFNTEKIRVPLGGSRGITFQVWDVG GQEKLRPLWRSYTRRTDGLVFVVDAAEAERLEEAKVELHRISRAS DNQ GVPVLVLANKQDQPGALSAAEVEKRLAVRELAAATLTHVQGCSAVDGLGL QQGLERLYEMILKRKKAARGGK KRR |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **文献名称**: "Characterization of Recombinant ARL4D and Its Role in Cilia Assembly"
**作者**: Smith J, et al.
**摘要**: 研究通过大肠杆菌系统重组表达ARL4D蛋白，证实其通过激活Rab11通路促进初级纤毛形成，揭示其在细胞器运输中的调控作用。
2. **文献名称**: "Structural Insights into ARL4D and Its Interaction with Effector Proteins"
**作者**: Lee H, et al.
**摘要**: 利用X射线晶体学解析ARL4D重组蛋白的GTP结合构象，发现其与BART蛋白复合物结合的结构基础，阐明ARL4D在膜转运中的分子机制。
3. **文献名称**: "ARL4D Regulates Neuronal Migration via Rho GTPase Signaling"
**作者**: Chen X, et al.
**摘要**: 通过重组ARL4D蛋白体外功能实验，证明其通过激活Cdc42调控神经元迁移，提示其在神经发育疾病中的潜在病理意义。
4. **文献名称**: "Expression and Purification of Functional ARL4D for Drug Screening"
**作者**: Tanaka R, et al.
**摘要**: 开发昆虫细胞系统高效表达ARL4D重组蛋白，建立基于其GTP酶活性的高通量药物筛选平台，用于靶向ARL4D的抑制剂开发。
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**说明**：以上文献为示例，实际需根据具体研究方向补充真实文献。建议通过PubMed或Google Scholar搜索关键词“ARL4D recombinant”“ARL4D function”获取最新研究。
**Background of ARL4D Recombinant Protein**
ARL4D (ADP-ribosylation factor-like 4D) is a small GTP-binding protein belonging to the ARL family, a subgroup within the RAS superfamily. These proteins are evolutionarily conserved and play critical roles in regulating intracellular trafficking, cytoskeletal dynamics, and membrane remodeling. Unlike classical ARF (ADP-ribosylation factor) proteins, ARL4D lacks ADP-ribosylation activity but retains GTPase functionality, enabling it to cycle between active (GTP-bound) and inactive (GDP-bound) states. This conformational switch allows ARL4D to interact with effector molecules, modulating downstream signaling pathways.
ARL4D is ubiquitously expressed, with notable roles in cell migration, neurite outgrowth, and organelle positioning. Studies highlight its involvement in cancer progression, particularly in tumor cell invasion and metastasis, as well as in neurodevelopmental processes. Its localization to the plasma membrane and endosomal compartments suggests a function in vesicle transport or receptor recycling. Dysregulation of ARL4D has been linked to pathologies such as colorectal cancer, Alzheimer’s disease, and ciliopathies.
Recombinant ARL4D protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to ensure high purity and functional activity. This tool enables mechanistic studies, including GTP-binding assays, protein interaction analyses, and structural investigations (e.g., X-ray crystallography). Researchers utilize it to map ARL4D’s interactome, decipher its regulatory networks, and explore therapeutic targeting strategies. Quality control involves validation via SDS-PAGE, Western blotting, and mass spectrometry to confirm identity and post-translational modifications.
Overall, ARL4D recombinant protein serves as a vital resource for unraveling its biological significance and translational potential in human health and disease.
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