首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人M型肌酸激酶(CKM)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CKM含量。CKM（心血管-肾脏-代谢综合征）是一种由肥胖、糖尿病、慢性肾脏疾病和心血管疾病相互作用的全身性疾病。其研究机制涉及代谢异常、炎症反应和脂肪组织功能紊乱，导致心血管和肾脏损伤。通过药物如SGLT2抑制剂、GLP-1受体激动剂等，可同时改善代谢、肾脏和心血管健康。试剂盒检测范围为3.12 μU/mL-200 μU/mL，科研人员可利用该产品探索肌肉损伤模型中的生物标志物动态变化，分析心血管疾病或代谢性疾病动物模型中能量代谢调控机制，亦可应用于运动医学研究或药物开发中评估干预措施对肌肉功能的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CKM ELISA Kit; CKMM ELISA Kit; Creatine kinase M ELISA Kit; Creatine kinase M chain ELISA Kit; Creatine kinase M type ELISA Kit; Creatine kinase M-type ELISA Kit; Creatine kinase muscle ELISA Kit; Creatine kinase, muscle type ELISA Kit; KCRM_HUMAN ELISA Kit; M-CK ELISA Kit; MCK ELISA Kit; Muscle creatine kinase ELISA Kit
缩写：CKM
Uniprot No.：P06732
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 μU/mL-200 μU/mL
灵敏度：1.56 μU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CKM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:100 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:200 | Average % | 98 | |||||
| Range % | 94-102 | ||||||
| 1:400 | Average % | 90 | |||||
| Range % | 86-94 | ||||||
| 1:800 | Average % | 106 | |||||
| Range % | 101-113 | ||||||
回收率：
| The recovery of human CKM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 90-100 | |||||
| EDTA plasma (n=4) | 89 | 85-93 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| μU/mlOD1OD2Average 2000.1550.1610.158 1000.2720.2600.266 500.4430.4550.449 250.7060.6820.694 12.51.0451.0071.026 6.251.2481.2931.271 3.121.6421.7141.678 02.0552.1592.107 | |||||||
货期：3-5 working days
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