首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人细胞骨架相关蛋白4(CKAP4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CKAP4含量。CKAP4是一种重要靶点。它在细胞内微管组织等方面发挥作用。相关研究围绕其在疾病发生发展中的机制展开，如在肿瘤细胞的增殖、迁移等过程里的影响，期望通过对其机制的深入探究，为开发针对性的治疗策略提供依据。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，可满足基础科研中细胞功能研究、疾病模型构建及药物干预效果评价等需求。适用于体外培养细胞、动物模型组织或体液样本的定量分析，为探索CKAP4在细胞增殖、凋亡、代谢调控等过程中的分子机制提供可靠工具，助力肿瘤生物学、分子病理学等领域的创新研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：63 kDa membrane protein ELISA Kit; CKAP 4 ELISA Kit; Ckap4 ELISA Kit; CKAP4_HUMAN ELISA Kit; Cytoskeleton associated protein 4 ELISA Kit; Cytoskeleton-associated protein 4 ELISA Kit; ERGIC 63 ELISA Kit; ERGIC63 ELISA Kit; MGC99554 ELISA Kit; p63 ELISA Kit; Transmembrane protein 63kD endoplasmic reticulum Golgi intermediate compartment ELISA Kit; Type II transmembrane protein p63 ELISA Kit
缩写：CKAP4
Uniprot No.：Q07065
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.44 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CKAP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 86 | |||||
| Range % | 83-89 | ||||||
| 1:2 | Average % | 95 | |||||
| Range % | 91-99 | ||||||
| 1:4 | Average % | 87 | |||||
| Range % | 83-90 | ||||||
| 1:8 | Average % | 99 | |||||
| Range % | 92-103 | ||||||
回收率：
| The recovery of human CKAP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 86 | 83-89 | |||||
| EDTA plasma (n=4) | 100 | 95-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected15002.3452.4012.3732.2007502.0132.1122.0631.8903751.7541.7041.7291.556187.51.3341.3041.3191.14693.750.8120.8310.8220.64946.880.5030.5120.5080.33523.440.3460.3380.3420.16900.1720.1740.173 | |||||||
货期：3-5 working days
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