Rat Keratin, type I cytoskeletal 18(KRT18) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EQ027319RA

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
大鼠细胞角蛋白18-M65（CK 18-M65）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆样本中的KRT18含量。KRT18 是角蛋白家族成员。在细胞中维持细胞结构稳定、参与信号传导。其异常表达与肿瘤等疾病密切相关。研究发现它可作为肿瘤诊断标志物，还参与肿瘤细胞增殖、迁移等过程，有望成为潜在治疗靶点。试剂盒检测范围为0.94 ng/mL-60 ng/mL，适用于科研领域中对大鼠模型的组织病理机制研究，例如肝纤维化、肝癌模型构建或化学诱导毒性实验中细胞死亡动态监测，为评估上皮细胞损伤程度及疾病进展提供定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Krt18 ELISA Kit; Krt1-18Keratin ELISA Kit; type I cytoskeletal 18 ELISA Kit; Cytokeratin-18 ELISA Kit; CK-18 ELISA Kit; Keratin-18 ELISA Kit; K18 ELISA Kit
缩写：
KRT18
Uniprot No.：
Q5BJY9
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma,tissue homogenates
检测范围：
0.94 ng/mL-60 ng/mL
灵敏度：
0.23 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat KRT18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-100
1:2	Average %	99
	Range %	94-103
1:4	Average %	101
	Range %	98-105
1:8	Average %	98
	Range %	93-104

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
60	2.606	2.657	2.632	2.469
30	1.798	1.774	1.786	1.623
15	1.152	1.198	1.175	1.012
7.5	0.713	0.739	0.726	0.563
3.75	0.446	0.468	0.457	0.294
1.87	0.334	0.348	0.341	0.178
0.94	0.265	0.267	0.266	0.103
0	0.161	0.165	0.163