Mouse Keratin, type I cytoskeletal 18(KRT18) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E17158m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠细胞角蛋白18（KTR18）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的KRT18含量。KRT18即角蛋白18，是细胞骨架中间丝的重要组成部分。其表达异常与多种疾病密切相关，如肿瘤发生发展。研究发现，它在细胞增殖、迁移及抗凋亡等方面发挥机制作用，有望成为疾病诊断标志物和治疗靶点，具有重要科研与临床价值。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于科研领域中对小鼠上皮细胞损伤、肝纤维化、肿瘤模型或炎症性疾病的研究，例如通过检测循环系统中KRT18水平评估细胞凋亡程度、疾病进展或药物干预效果，为分子机制探索及生物标志物分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Krt18 ELISA Kit; Kerd ELISA Kit; Krt1-18 ELISA Kit; Keratin ELISA Kit; type I cytoskeletal 18 ELISA Kit; Cytokeratin endo B ELISA Kit; Keratin D ELISA Kit; Cytokeratin-18 ELISA Kit; CK-18 ELISA Kit; Keratin-18 ELISA Kit; K18 ELISA Kit
缩写：
KRT18
Uniprot No.：
P05784
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma
检测范围：
0.156 ng/mL-10 ng/mL
灵敏度：
0.039 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse KRT18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	83
	Range %	80-86
1:2	Average %	93
	Range %	89-97
1:4	Average %	84
	Range %	81-89
1:8	Average %	100
	Range %	95-105

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
10	2.199	2.387	2.293	2.122
5	1.502	1.538	1.520	1.349
2.5	0.791	0.788	0.790	0.619
1.25	0.430	0.468	0.449	0.278
0.625	0.302	0.312	0.307	0.136
0.312	0.252	0.256	0.254	0.083
0.156	0.201	0.210	0.206	0.035
0	0.172	0.170	0.171