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产品描述：大鼠细胞角蛋白18片段Asp396(K18-Asp396)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CK 18M30含量。试剂盒检测范围为1.56 mIU/mL-100 mIU/mL，适用于基础科研中动态监测细胞凋亡水平、评估药物干预效果或探究疾病模型中上皮细胞损伤机制。适用于肝脏疾病模型研究、肿瘤生物学实验或毒理学评估等非临床科研场景，为细胞死亡相关分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：CK 18M30
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 mIU/mL-100 mIU/mL
灵敏度：0.39 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CK 18-M30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 97 | |
| Range % | 91-102 | ||
| 1:2 | Average % | 94 | |
| Range % | 90-98 | ||
| 1:4 | Average % | 84 | |
| Range % | 80-88 | ||
| 1:8 | Average % | 103 | |
| Range % | 99-112 |
回收率：
| The recovery of rat CK 18-M30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-94 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected 1002.5992.5122.5562.407 501.7691.7211.7451.596 251.0661.0241.0450.896 12.50.6070.6550.6310.482 6.250.3580.3890.3740.225 3.120.2790.2950.2870.138 1.560.2130.2090.2110.062 00.1540.1430.149 | ||||||
货期：3-5 working days
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