首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人细胞角蛋白18片段Asp396(K18-Asp396)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物.样本中的CK 18M30含量。试剂盒检测范围为15.6 mIU/ml-1000 mIU/ml，适用于体外研究细胞凋亡信号通路、评估药物肝毒性引起的上皮细胞损伤、探索炎症性疾病中组织修复机制等科研实验本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：CK 18M30
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates.
检测范围：15.6 mIU/ml-1000 mIU/ml
灵敏度：3.9 mIU/ml
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CK 18-M30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||
| Sample | Serum(n=4) | ||
| 1:1 | Average % | 90 | |
| Range % | 85-94 | ||
| 1:2 | Average % | 86 | |
| Range % | 81-92 | ||
| 1:4 | Average % | 94 | |
| Range % | 90-99 | ||
| 1:8 | Average % | 89 | |
| Range % | 83-96 |
回收率：
| The recovery of human CK 18-M30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 99 | 94-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected 10002.5772.5522.5652.443 5002.0562.0162.0361.914 2501.2671.2021.2351.113 1250.6830.6480.6660.544 62.50.3790.4050.3920.270 31.20.2940.2790.2870.165 15.60.2010.1990.2000.078 00.1170.1260.122 | ||||||
货期：3-5 working days
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