关键信息
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基因名
PDE7A1
- 应用
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别名
High affinity 3',5'-cyclic-AMP phosphodiesterase 7A; HCP1; TM22; cAMP-specific phosphodiesterase 7A; PDE7A; Homo sapiens; Human; Hydrolase; 3.1.4.53
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种属
Human
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表达系统
E. coli
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标签
Tag Free
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q13946-2
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表达区间
S130-S482
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蛋白长度
Partial
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PDE7A1, or phosphodiesterase 7A1, is an important enzyme that plays a crucial role in the regulation of intracellular levels of cyclic nucleotides, specifically cyclic AMP (cAMP), which is pivotal in various cellular signaling pathways. Research into PDE7A1 has gained attention due to its implications in several physiological and pathological processes, including inflammation, neuronal signaling, and metabolic regulation. Dysregulation of cAMP levels has been associated with various diseases, including asthma, depression, and certain cancers. Consequently, PDE7A1 is considered a potential therapeutic target for drug development aimed at modulating cAMP signaling. The focus on recombinant protein studies of PDE7A1 allows for a deeper understanding of its structure, function, and the mechanisms governing its activity. By producing and characterizing recombinant PDE7A1, researchers can investigate the enzyme's kinetic properties, inhibitor binding, and interactions with other cellular components. This knowledge could lead to the development of selective inhibitors or modulators with therapeutic potential. Overall, the study of recombinant PDE7A1 is essential for translating basic research into clinical applications, providing insights into the enzyme's role in health and disease.
