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产品描述：人冷诱导RNA结合蛋白(CIRBP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CIRBP含量。CIRBP即冷诱导RNA结合蛋白，在细胞应激反应等方面具有重要作用。研究发现它能结合特定RNA序列调节基因表达。其表达异常与多种疾病相关，如肿瘤、神经退行性疾病等，深入研究其机制或为疾病治疗提供新思路。试剂盒检测范围为12.5 pg/mL-800 pg/mL，该产品适用于低温应激相关分子机制研究、细胞周期调控实验，或炎症性疾病及肿瘤微环境等领域的蛋白表达水平检测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A18 hnRNP ELISA Kit; A18HNRNP ELISA Kit; cirbp ELISA Kit; CIRBP_HUMAN ELISA Kit; CIRP ELISA Kit; Cold inducible RNA binding protein ELISA Kit; Cold-inducible RNA-binding protein ELISA Kit; Glycine rich RNA binding protein ELISA Kit; Glycine-rich RNA-binding protein CIRP ELISA Kit; testicular tissue protein Li 39 ELISA Kit
缩写：CIRBP
Uniprot No.：Q14011
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CIRBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:10 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:20 | Average % | 89 | ||||
| Range % | 85-95 | |||||
| 1:40 | Average % | 92 | ||||
| Range % | 86-99 | |||||
回收率：
| The recovery of human CIRBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 84-99 | ||||
| EDTA plasma (n=4) | 98 | 90-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
 pg/mlOD1OD2AverageCorrected8002.1152.2152.1652.0244001.6171.7171.6671.5262001.0571.0171.0370.8961000.6920.7020.6970.556500.3980.4080.4030.262250.3110.3210.3160.17512.50.1990.1950.1970.05600.1410.1400.141 | ||||||
货期：3-5 working days
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