首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人软骨中间层蛋白1(CILP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CILP含量。CILP（Contrastive Language-Image Pre-Training）是一种由OpenAI提出的预训练模型，旨在通过对比学习预训练语言和图像的表示，以实现图像和文本之间的相互理解。该模型通过大量图像与文本对进行预训练，采用自监督学习方法，通过度量图像与文本之间的相似性来提高模型的表现能力。试剂盒检测范围为93.75 pg/mL-6000 pg/mL，该产品适用于体外研究软骨组织工程模型中的基质代谢调控、退行性关节疾病动物模型的生物标志物追踪，以及临床前研究中的软骨修复效果评估等科研场景，为探索软骨病变机制及潜在干预策略提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cartilage intermediate layer protein 1 ELISA Kit; Cartilage intermediate layer protein 1 C1 ELISA Kit; Cartilage intermediate layer protein 1 C2 ELISA Kit; cartilage intermediate layer protein ELISA Kit; cartilage intermediate layer protein; nucleotide pyrophosphohydrolase ELISA Kit; Cartilage intermediate-layer protein ELISA Kit; CILP 1 ELISA Kit; CILP ELISA Kit; CILP-1 ELISA Kit; CILP1_HUMAN ELISA Kit; HGNC:1980 ELISA Kit; HsT18872 ELISA Kit
缩写：CILP
Uniprot No.：O75339
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：93.75 pg/mL-6000 pg/mL
灵敏度：23.4 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CILP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:10 | Average % | 105 | ||||
| Range % | 100-112 | |||||
| 1:20 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:40 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human CILP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-98 | ||||
| EDTA plasma (n=4) | 98 | 94-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected60002.0982.2012.1502.02730001.5121.4541.4831.36015000.9830.9340.9590.8367500.6110.6430.6270.5043750.3300.3380.3340.211187.50.2820.3020.2920.16993.750.1920.2070.2000.07700.1250.1200.123 | ||||||
货期：3-5 working days
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