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产品描述：人软骨间层蛋白2(CILP2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CILP2含量。试剂盒检测范围为25 pg/mL-1600 pg/mL，本试剂盒通过高特异性配对抗体实现精准捕获与信号放大，适用于软骨相关疾病模型研究、药物干预效果评估以及生物标志物筛选等科研场景。支持多种生物样本类型检测，实验操作全程无需特殊设备，配套标准品与预包被板条可满足实验室常规检测需求，为软骨生物学、运动医学及再生医学领域的基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CILP2 ELISA Kit; Cartilage intermediate layer protein 2 ELISA Kit; CILP-2) [Cleaved into: Cartilage intermediate layer protein 2 C1; Cartilage intermediate layer protein 2 C2] ELISA Kit
缩写：CILP2
Uniprot No.：Q8IUL8
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：25 pg/mL-1600 pg/mL
灵敏度：6.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CILP2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 91-103 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of human CILP2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 84-93 | ||||
| EDTA plasma (n=4) | 90 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 16002.1531.9842.0691.986 8001.7471.7121.7301.647 4001.3871.3011.3441.261 2000.9650.9390.9520.869 1000.6430.6040.6240.541 500.3110.2890.3000.217 250.1920.1790.1860.103 00.0850.0800.083 | ||||||
货期：3-5 working days
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