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Human Chitinase-3-like protein 2(CHI3L2) ELISA kit

  • 中文名: 人几丁质酶3样蛋白2(CHI3L2)酶联免疫试剂盒
货号: IPD-EL005347HU
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人几丁质酶3样蛋白2(CHI3L2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CHI3L2含量。CHI3L2是一种糖基水解酶家族成员,缺乏酶活性,通过结合多糖和细胞表面受体调控炎症、组织重塑和肿瘤微环境。在关节炎、纤维化疾病和恶性肿瘤中高表达,是疾病进展的生物标志物和潜在治疗靶点。试剂盒检测范围为0.78 ng/ml-50 ng/ml,该产品可为炎症性疾病机制研究、纤维化模型评估或肿瘤微环境分析提供关键生物标志物检测支持,特别适用于基础医学、免疫学及分子病理学领域的体外样本检测,帮助研究者探索CHI3L2在疾病发生发展中的调控作用。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    CH3L2_HUMAN ELISA Kit; CHI3L2 ELISA Kit; Chitinase 3 like 2 ELISA Kit; Chitinase-3-like protein 2 ELISA Kit; Chondrocyte protein 39 ELISA Kit; OTTHUMP00000014028 ELISA Kit; YKL 39 ELISA Kit; YKL-39 ELISA Kit; YKL39 ELISA Kit
  • 缩写:
    CHI3L2
  • Uniprot No.:
    Q15782
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.78 ng/ml-50 ng/ml
  • 灵敏度:
    0.19 ng/ml
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Stem Cells
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human CHI3L2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    87


    Range %

    81-91


    1:2

    Average %

    101


    Range %

    95-105


    1:4

    Average %

    102


    Range %

    98-109


    1:8

    Average %

    96


    Range %

    91-98


  • 回收率:

    The recovery of human CHI3L2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    94

    89-96


    EDTA plasma (n=4)

    96

    91-98


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    50

    2.701

    2.688

    2.695

    2.582


    25

    2.344

    2.192

    2.268

    2.155


    12.5

    1.704

    1.729

    1.717

    1.604


    6.25

    1.151

    1.137

    1.144

    1.031


    3.125

    0.678

    0.699

    0.689

    0.576


    1.56

    0.388

    0.366

    0.377

    0.264


    0.78

    0.238

    0.241

    0.240

    0.127


    0

    0.115

    0.111

    0.113




  • 货期:
    3-5 working days

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