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产品描述：小鼠几丁质酶3样蛋白1(CHI3L1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CHI3L1含量。CHI3L1是一个重要的靶点。它在生理和病理过程中发挥作用，与炎症、纤维化等密切相关。研究机制方面，主要聚焦于其在细胞信号传导、免疫调节等方面的功能，探索其在疾病发生发展中的作用及潜在的治疗干预方式。试剂盒检测范围为0.47 ng/mL-30 ng/mL，本试剂盒适用于科研场景中探索CHI3L1在疾病模型中的表达动态，例如评估炎症性疾病或纤维化动物模型中该蛋白的分泌水平变化，亦可应用于体外细胞实验中对药物干预或基因调控后CHI3L1释放量的定量分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Chi3l1 ELISA Kit; Brp39 ELISA Kit; Chil1 ELISA Kit; Chitinase-3-like protein 1 ELISA Kit; BRP39 protein ELISA Kit; Breast regression protein 39 ELISA Kit; Cartilage glycoprotein 39 ELISA Kit; CGP-39 ELISA Kit; GP-39 ELISA Kit
缩写：CHI3L1
Uniprot No.：Q61362
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.47 ng/mL-30 ng/mL
灵敏度：0.11 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse CHI3L1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 84 | |||||
| Range % | 80-88 | ||||||
| 1:2 | Average % | 91 | |||||
| Range % | 86-95 | ||||||
| 1:4 | Average % | 103 | |||||
| Range % | 99-107 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 85-98 | ||||||
回收率：
| The recovery of mouse CHI3L1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-93 | |||||
| EDTA plasma (n=4) | 95 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected302.6012.4762.5392.319151.9331.8191.8761.6567.51.1401.2041.1720.9523.750.6920.6820.6870.4671.880.4570.4410.4490.2290.940.3490.3580.3540.1340.470.2840.2970.2910.07100.2240.2150.220 | |||||||
货期：3-5 working days
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