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产品描述：人嗜铬蛋白A(CgA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、尿液、脑脊液（CSF）样本中的CHGA含量。CHGA 即嗜铬粒蛋白 A，是一种酸性糖蛋白。它广泛分布于神经内分泌细胞的分泌颗粒中。在研究机制方面，它可被酶解产生多种生物活性肽，参与血压调节、免疫调节等生理过程，在肿瘤、心血管疾病等研究中备受关注。试剂盒检测范围为62.5 ng/mL-4000 ng/mL，适用于神经内分泌调控机制研究、疾病相关生物标志物探索、药物干预效果评估等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：beta Granin ELISA Kit; betagranin (N-terminal fragment of chromogranin A) ELISA Kit; catestatin ELISA Kit; CgA ELISA Kit; CHG A ELISA Kit; Chga ELISA Kit; chromofungin ELISA Kit; Chromogranin A ELISA Kit; Chromogranin A parathyroid secretory protein 1 ELISA Kit; Chromogranin A precursor ELISA Kit; ChromograninA ELISA Kit; CMGA_HUMAN ELISA Kit; ER-37 ELISA Kit; Pancreastatin ELISA Kit; Parastatin ELISA Kit; Parathyroid secretory protein 1 ELISA Kit; Pituitary secretory protein I ELISA Kit; Secretory protein I ELISA Kit; SP I ELISA Kit; SP-I ELISA Kit; SP1 ELISA Kit; SPI ELISA Kit; vasostatin 2 ELISA Kit; Vasostatin ELISA Kit; Vasostatin I ELISA Kit; Vasostatin II ELISA Kit; vasostatin-2 ELISA Kit
缩写：CHGA
Uniprot No.：P10645
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, urine, cerebrospinal fluid (CSF)
检测范围：62.5 ng/mL-4000 ng/mL
灵敏度：15.6 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 83-89 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 106 | ||||
| Range % | 102-110 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 95-99 | |||||
回收率：
| The recovery of human CgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 95-104 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 40002.6142.5982.6062.421 20002.0542.0782.0661.881 10001.5831.5421.5631.378 5001.0051.0941.0500.865 2500.6680.6780.6730.488 1250.4820.5010.4920.307 62.50.3470.3680.3580.173 00.1840.1860.185 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human CgA antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled CgA antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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