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产品描述：人胆固醇酯转移蛋白(CETP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CETP含量。试剂盒检测范围为0.78 ng/mL-50 ng/mL，本产品适用于体外定量分析多种生物样本中的CETP浓度，如探究疾病模型中CETP的表达变化、评估药物或基因调控对胆固醇代谢通路的影响等本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BPIFF ELISA Kit; CETP ELISA Kit; CETP_HUMAN ELISA Kit; Cholesteryl ester transfer protein ELISA Kit; Cholesteryl ester transfer protein plasma ELISA Kit; HDLCQ10 ELISA Kit; Lipid transfer protein I ELISA Kit
缩写：CETP
Uniprot No.：P11597
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CETP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 89 | ||||
| Range % | 84-94 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:400 | Average % | 104 | ||||
| Range % | 100-108 | |||||
| 1:800 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of human CETP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 94-96 | ||||
| EDTA plasma (n=4) | 101 | 95-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.1672.1992.1832.030251.7081.7591.7341.58112.51.2811.2981.2901.1376.250.8620.8910.8770.7243.120.5900.5630.5770.4241.560.4360.4050.4210.2680.780.2470.2530.2500.09700.1540.1520.153 | ||||||
货期：3-5 working days
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