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产品描述:人牙骨质蛋白1(CEMP-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆样本中的CEMP1含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL,该产品适用于体外实验研究,可用于探索牙周炎病理机制、评估牙骨质再生治疗方案的疗效,或作为骨代谢相关疾病的分子机制研究工具,为口腔医学、骨生物学等领域的科研工作者提供精准的蛋白定量解决方案。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Cementoblastoma-derived protein 1 ELISA Kit; Cementum protein 1 ELISA Kit; Cementum protein 23 ELISA Kit; CEMP1 ELISA Kit; CEMP1_HUMAN ELISA Kit; CP-23 ELISA Kit; CP23 ELISA Kit
缩写:CEMP1
Uniprot No.:Q6PRD7
种属:Homo sapiens (Human)
样本类型:serum, plasma
检测范围:0.156 ng/mL-10 ng/mL
灵敏度:0.039 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cell Biology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human CEMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 90 | ||||
Range % | 85-95 | |||||
1:2 | Average % | 101 | ||||
Range % | 97-105 | |||||
1:4 | Average % | 83 | ||||
Range % | 80-86 | |||||
1:8 | Average % | 107 | ||||
Range % | 103-110 |
回收率:
The recovery of human CEMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 92 | 87-96 | ||||
EDTA plasma (n=4) | 91 | 84-97 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2AverageCorrected102.6582.6972.6782.52852.3122.3312.3222.1722.51.8711.8921.8821.7321.251.3121.3651.3391.1890.6250.7880.7970.7930.6430.3120.4420.4720.4570.3070.1560.2810.2720.2770.12700.1510.1490.150 |
货期:3-5 working days
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