| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | FBS; DC10; FBX8 |
| WB Predicted band size | 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human FBXO8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于FBXO8抗体的模拟参考文献示例(内容为假设性概括，实际文献需通过学术数据库核实)：
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1. **文献名称**: "FBXO8 regulates neuronal proteostasis through ubiquitin-mediated degradation pathways"
**作者**: Kumar A, et al.
**摘要**: 本研究利用特异性FBXO8抗体，揭示了FBXO8在神经细胞中通过泛素-蛋白酶体系统调控异常蛋白聚集的作用，与阿尔茨海默病模型中的病理过程相关。抗体验证显示其在脑组织中的差异表达。
2. **文献名称**: "FBXO8 interacts with p53 to modulate DNA damage response in cancer cells"
**作者**: Li Y, et al.
**摘要**: 通过免疫共沉淀(使用FBXO8抗体)和功能实验，研究发现FBXO8与p53蛋白直接结合，促进其泛素化降解，从而影响癌细胞对化疗的敏感性，为靶向治疗提供潜在策略。
3. **文献名称**: "Structural characterization of FBXO8 and its role in substrate recognition"
**作者**: Suzuki M, et al.
**摘要**: 利用抗FBXO8抗体进行免疫荧光和蛋白质印迹分析，解析了FBXO8的F-box结构域与底物结合机制，证明其通过特定基序识别泛素化靶标，调控细胞周期进程。
4. **文献名称**: "FBXO8 downregulation promotes gastric cancer metastasis via EMT activation"
**作者**: Wang X, et al.
**摘要**: 研究通过FBXO8抗体检测临床样本，发现FBXO8低表达与胃癌患者不良预后相关，机制上通过抑制上皮-间质转化(EMT)通路减缓肿瘤转移。
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**注意**：以上文献信息为示例，实际引用时需通过PubMed、Google Scholar等平台检索真实文献(关键词：FBXO8 antibody, FBXO8 function)。若需实验抗体信息，可查阅抗体公司(如CST、Abcam)的产品说明书及相关引用文献。
The FBXO8 antibody is a tool used to detect and study F-box protein 8 (FBXO8), a member of the F-box protein family. F-box proteins are critical components of the SKP1-CUL1-F-box (SCF) ubiquitin ligase complex, which targets specific substrates for ubiquitination and subsequent proteasomal degradation. FBXO8 contains an F-box domain that facilitates interaction with SKP1. enabling substrate recognition via its C-terminal domain. It plays roles in cellular processes such as cell cycle regulation, DNA repair, and signal transduction, though its precise substrates and mechanisms remain under investigation.
Antibodies against FBXO8 are commonly used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to analyze its expression, localization, and interactions in various biological contexts. These antibodies are essential for studying FBXO8's involvement in diseases, including cancer and neurodegenerative disorders, where ubiquitination pathways are often dysregulated. Researchers also use FBXO8 antibodies to explore tissue-specific expression patterns and post-translational modifications.
Challenges in working with FBXO8 antibodies include ensuring specificity, as F-box proteins share structural similarities. Validation via knockout/knockdown controls or mass spectrometry is recommended. Commercially available antibodies may vary in clonality (polyclonal or monoclonal) and target epitopes (e.g., N-terminal vs. C-terminal regions), influencing experimental outcomes. Proper storage and optimized dilution protocols are critical for reproducibility. Overall, FBXO8 antibodies are vital for unraveling its biological functions and therapeutic potential.
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