首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人CD9抗原(CD9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CD9含量。CD9是一种跨膜四超家族成员，属于四跨膜蛋白家族，具有四个跨膜结构域，参与细胞分化、粘附和信号转导等过程。CD9在细胞粘附、细胞运动、肿瘤转移以及精卵融合等方面发挥重要作用，其研究机制涉及与整合素、糖皮质激素受体等的相互作用。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于基础研究中对CD9功能的探索，例如在肿瘤生物学中分析其与转移潜能的关联，或在免疫学研究中评估其在细胞间囊泡（如外泌体）中的作用机制，为体外实验及疾病模型研究提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD9; MIC3; TSPAN29; GIG2; CD9 antigen; 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24; CD antigen CD9
缩写：CD9
Uniprot No.：P21926
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 81-90 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 91-110 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 84-98 | |||||
回收率：
| The recovery of human CD9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 81-98 | ||||
| EDTA plasma (n=4) | 92 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.2092.1522.1812.030 51.7101.7061.7081.557 2.51.2441.2041.2241.073 1.250.8600.8390.8500.699 0.6250.4930.4590.4760.325 0.3120.3200.2950.3080.157 0.1560.2410.2290.2350.084 00.1540.1470.151 | ||||||
货期：3-5 working days
×
