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产品描述：人CD8分子(CD8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CD8A含量。CD8A是一个重要靶点。它与免疫功能密切相关，CD8A阳性T细胞在机体免疫防御、抗肿瘤等方面发挥重要作用。研究机制主要聚焦于调节CD8 + T细胞的活化、增殖与功能等，以增强免疫反应，为肿瘤、感染性疾病等治疗提供新思路。试剂盒检测范围为62.5 U/mL-4000 U/mL，适用于免疫学基础研究、疾病模型分析（如肿瘤微环境评估或感染性疾病免疫反应监测）以及药物开发中T细胞活性相关指标的检测。可通过检测外周血样本评估机体免疫状态，或利用组织匀浆分析局部CD8+ T细胞浸润程度，为探索免疫调节机制、评估疫苗或免疫治疗方案的科研需求提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：alpha polypeptide (p32) ELISA Kit; CD8 ELISA Kit; CD8 antigen alpha polypeptide ELISA Kit; CD8 antigen alpha polypeptide (p32) ELISA Kit; CD8a ELISA Kit; CD8A antigen ELISA Kit; CD8A molecule ELISA Kit; CD8A_HUMAN ELISA Kit; Leu2 ELISA Kit; Leu2 T lymphocyte antigen ELISA Kit; Ly3 ELISA Kit; LYT3 ELISA Kit; MAL ELISA Kit; OKT8 T cell antigen ELISA Kit; OTTHUMP00000160760 ELISA Kit; OTTHUMP00000160764 ELISA Kit; OTTHUMP00000203528 ELISA Kit; OTTHUMP00000203721 ELISA Kit; p32 ELISA Kit; T cell antigen Leu2 ELISA Kit; T cell co receptor ELISA Kit; T-cell surface glycoprotein CD8 alpha chain ELISA Kit; T-lymphocyte differentiation antigen T8/Leu-2 ELISA Kit; T8 T cell antigen ELISA Kit; T8/Leu-2 T-lymphocyte differentiation antigen ELISA Kit
缩写：CD8A
Uniprot No.：P01732
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：62.5 U/mL-4000 U/mL
灵敏度：15.6 U/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 94 | |||||
| Range % | 86-98 | ||||||
| 1:2 | Average % | 99 | |||||
| Range % | 88-104 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 89-100 | ||||||
| 1:8 | Average % | 105 | |||||
| Range % | 94-109 | ||||||
回收率：
| The recovery of human CD8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 100 | 88-104 | |||||
| EDTA plasma (n=4) | 97 | 88-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| U/mlOD1OD2AverageCorrected 40001.9791.9411.9601.783 20001.4711.4551.4631.286 10000.9610.9890.9750.798 5000.6470.6850.6660.489 2500.4470.4540.4510.274 1250.2910.3080.3000.123 62.50.2270.2320.2300.053 00.1790.1750.177 | |||||||
货期：3-5 working days
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