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产品描述：人可溶性CD86(B7-2/sCD86)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD86含量。CD86 是重要的免疫共刺激分子，在免疫应答激活中发挥关键作用。其它表达于抗原提呈细胞表面。研究机制上，能与 T 细胞表面受体结合，为 T 细胞活化提供第二信号，增强 T 细胞功能，是免疫治疗领域的潜在靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品适用于免疫调控机制研究、炎症性疾病模型评估、肿瘤免疫治疗相关基础研究等领域，为探索CD86在T细胞活化、免疫耐受及病理状态中的作用提供可靠的检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD86; CD28LG2; T-lymphocyte activation antigen CD86; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD antigen CD86
缩写：CD86
Uniprot No.：P42081
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human sCD86 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 91-95 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-101 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 92-106 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 82-90 | |||||
回收率：
| The recovery of human sCD86 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 89-95 | ||||
| EDTA plasma (n=4) | 103 | 100-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.5002.6592.5802.425102.1672.0352.1011.94651.4231.5441.4841.3292.50.8880.8520.8700.7151.250.6220.6310.6270.4720.6250.4140.4370.4260.2710.3120.2680.2810.2750.12000.1490.1610.155 | ||||||
货期：3-5 working days
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