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产品描述：人CD82分子酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的CD82含量。CD82 是一个研究靶点。它广泛参与细胞活动，如细胞迁移、黏附等。在癌症研究中，它常被视为抑癌因子，可抑制肿瘤细胞的侵袭和转移。其具体作用机制是通过与其他分子相互作用，调节信号通路，影响细胞的生物学行为 。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品可为肿瘤微环境研究、免疫细胞功能分析、炎症性疾病机制探索等基础科研提供量化依据，尤其适用于肿瘤生物学、干细胞迁移调控及免疫代谢相关研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD82; KAI1; SAR2; ST6; TSPAN27; CD82 antigen; C33 antigen; IA4; Inducible membrane protein R2; Metastasis suppressor Kangai-1; Suppressor of tumorigenicity 6 protein; Tetraspanin-27; Tspan-27; CD antigen CD82
缩写：CD82
Uniprot No.：P27701
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD82 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:8 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of human CD82 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 99-110 | ||||
| EDTA plasma (n=4) | 89 | 82-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.7482.7022.7252.60152.1312.1922.1622.0382.51.4911.4161.4541.3301.250.8660.8270.8470.7230.6250.5460.5020.5240.4000.3120.3630.3910.3770.2530.1560.2540.2390.2470.12300.1250.1230.124 | ||||||
货期：3-5 working days
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