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产品描述：人可溶性CD80 (sCD80)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的CD80含量。CD80是一种重要的免疫共刺激分子，在免疫系统中起关键作用，可增强T细胞活化和免疫反应。其研究机制主要围绕调节免疫信号传导展开，科学家试图通过调控CD80的活性或表达，来治疗癌症、自身免疫病等，为免疫治疗提供新思路。试剂盒检测范围为125 pg/mL-8000 pg/mL，适用于体外实验中免疫应答机制研究、药物干预效果评估或疾病相关生物标志物探索；为科研人员研究sCD80在免疫调控网络中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD80; CD28LG; CD28LG1; LAB7; T-lymphocyte activation antigen CD80; Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1; B7; CD antigen CD80
缩写：CD80
Uniprot No.：P33681
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：125 pg/mL-8000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human sCD80 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 103 | |||||
| Range % | 99-107 | ||||||
| 1:2 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:4 | Average % | 96 | |||||
| Range % | 92-100 | ||||||
| 1:8 | Average % | 90 | |||||
| Range % | 85-95 | ||||||
回收率：
| The recovery of human sCD80 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 85 | 80-89 | |||||
| EDTA plasma (n=4) | 94 | 90-98 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected80002.6182.4852.5522.43540001.8051.6931.7491.63220001.0041.0471.0260.90910000.5470.5620.5550.4385000.3510.3340.3430.2262500.2300.2190.2250.1081250.1620.1710.1670.05000.1130.1200.117 | |||||||
货期：3-5 working days
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