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产品描述：人CD81抗原(CD81)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CD81含量。CD81是一种四次跨膜蛋白，属于Tetraspanins/TM4SF超家族，主要表达在多种组织细胞表面。它在病毒感染、细胞形态变化和肿瘤发生转移中发挥重要作用。CD81作为丙型肝炎病毒（HCV）的关键受体，参与病毒进入宿主细胞的过程，同时也是多种癌症的潜在靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础科研中探究CD81在免疫疾病、病毒感染或肿瘤微环境中的表达变化，或用于细胞信号通路、病原体宿主相互作用等分子机制研究，为体外实验样本的蛋白定量分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：26 kDa cell surface protein TAPA 1 ELISA Kit; 26 kDa cell surface protein TAPA-1 ELISA Kit; 26 kDa cell surface protein TAPA1 ELISA Kit; CD 81 ELISA Kit; CD81 ELISA Kit; CD81 antigen (target of antiproliferative ELISA Kit 1) ELISA Kit; CD81 antigen ELISA Kit; CD81 molecule ELISA Kit; CD81_HUMAN ELISA Kit; CVID6 ELISA Kit; S5.7 ELISA Kit; TAPA 1 ELISA Kit; TAPA1 ELISA Kit; Target of the antiproliferative ELISA Kit 1 ELISA Kit; Tetraspanin 28 ELISA Kit; Tetraspanin-28 ELISA Kit; Tetraspanin28 ELISA Kit; Tspan 28 ELISA Kit; Tspan-28 ELISA Kit; Tspan28 ELISA Kit
缩写：CD81
Uniprot No.：P60033
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD81 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-101 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-100 | |||||
| 1:5 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human CD81 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 95-100 | ||||
| EDTA plasma (n=4) | 98 | 94-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 102.1852.0642.1251.977 51.4071.3811.3941.246 2.50.8070.7900.7990.651 1.250.4510.4340.4430.295 0.6250.3260.3520.3390.191 0.3120.2690.2730.2710.123 0.1560.1970.2040.2010.053 00.1490.1460.148 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Human CD81antibody.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled CD81 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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