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产品描述：人HLA II类组织相容性抗原γ链(CD74)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD74含量。CD74是一种重要的靶点。它在免疫调节等生理过程中发挥作用。相关研究机制主要聚焦于其在抗原呈递等环节的功能，探索其与疾病发生发展的关联，期望通过对CD74的干预，为开发治疗特定疾病如癌症等的新疗法提供方向。试剂盒检测范围为0.9 ng/mL-60 ng/mL，适用于科研领域中对CD74蛋白表达动态的定量分析，例如研究免疫细胞活化状态、肿瘤微环境中的抗原呈递机制，或探索炎症性疾病中免疫调控网络的分子机制。可广泛应用于基础免疫学研究、药物作用机制探索及疾病模型构建等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD74; DHLAG; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; CD antigen CD74
缩写：CD74
Uniprot No.：P04233
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.9 ng/mL-60 ng/mL
灵敏度：0.225 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD74 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 97-108 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 85-92 | |||||
回收率：
| The recovery of human CD74 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-95 | ||||
| EDTA plasma (n=4) | 97 | 90-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected602.3112.4802.3962.260301.8651.9771.9211.785151.5861.4821.5341.3987.50.9991.0151.0070.8713.750.6320.6140.6230.4871.80.3740.3810.3780.2420.90.2650.2470.2560.12000.1340.1370.136 | ||||||
货期：3-5 working days
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