首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人CD73分子酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD73含量。CD73是细胞表面表达的一种酶，能催化AMP生成腺苷，在肿瘤微环境中发挥重要作用。其高表达与肿瘤免疫逃逸、血管生成和转移相关。研究机制主要围绕抑制CD73活性，阻断腺苷生成，激活免疫系统杀伤肿瘤细胞，有望成为新的肿瘤治疗靶点。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于腺苷信号通路研究、免疫细胞功能分析、肿瘤微环境标志物检测等科研场景，尤其为探索CD73在实体瘤组织中的表达特征、炎症性疾病中的免疫调控机制以及药物干预效果评估提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：5' NT ELISA Kit; 5' nucleotidase (CD73) ELISA Kit; 5' nucleotidase precursor ELISA Kit; 5' nucleotidase; ecto ELISA Kit; 5' nucleotidase; ecto (CD73) ELISA Kit; 5'-NT ELISA Kit; 5'-nucleotidase ELISA Kit; 5NTD_HUMAN ELISA Kit; CD73 ELISA Kit; CD73 antigen ELISA Kit; E5NT ELISA Kit; Ecto 5' nucleotidase ELISA Kit; Ecto-5'-nucleotidase ELISA Kit; eN ELISA Kit; eNT ELISA Kit; NT ELISA Kit; NT5 ELISA Kit; NT5E ELISA Kit; NTE ELISA Kit; Purine 5 Prime Nucleotidase ELISA Kit
缩写：CD73
Uniprot No.：P21589
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD73 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 81-91 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 93-103 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 95-103 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 88-98 | |||||
回收率：
| The recovery of human CD73 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-98 | ||||
| EDTA plasma (n=4) | 96 | 93-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.4982.4822.4902.3631001.9321.8171.8751.748501.2761.2561.2661.139250.7270.7140.7210.59412.50.4680.4440.4560.3296.250.3430.3210.3320.2053.120.2220.2120.2170.09000.1290.1250.127 | ||||||
货期：3-5 working days
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