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产品描述：人B细胞分化蛋白CD72(CD72)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD72含量。CD72是重要靶点，它是B细胞表面糖蛋白，在B细胞发育、活化及免疫调节中起关键作用。研究机制方面，聚焦于其与配体相互作用，通过信号通路影响B细胞功能，针对它的研究有望为免疫相关疾病如自身免疫病、肿瘤等治疗带来新策略。试剂盒检测范围为0.9 ng/mL-60 ng/mL，适用于科研领域探究B细胞相关免疫机制，如系统性红斑狼疮、类风湿性关节炎等自身免疫疾病模型中的B细胞功能研究，或肿瘤微环境中B淋巴细胞活化状态的分子机制分析，为免疫调控网络研究提供定量检测方案。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD72; B-cell differentiation antigen CD72; Lyb-2; CD antigen CD72
缩写：CD72
Uniprot No.：P21854
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.9 ng/mL-60 ng/mL
灵敏度：0.225 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 98 | |||||
| Range % | 92-103 | ||||||
| 1:2 | Average % | 91 | |||||
| Range % | 86-98 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 88-97 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 96-15 | ||||||
回收率：
| The recovery of human CD72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 90 | 87-95 | |||||
| EDTA plasma (n=4) | 101 | 103-106 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected602.5882.7872.6882.535302.1692.2362.2032.050151.6741.7441.7091.5567.51.3221.2231.2731.1203.750.7520.7440.7480.5951.80.3630.3750.3690.2160.90.2610.2550.2580.10500.1490.1560.153 | |||||||
货期：3-5 working days
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