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产品描述：人CD70抗原(CD70)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CD70含量。CD70是重要靶点，在免疫应答中发挥作用。其与受体CD27结合参与T、B淋巴细胞的活化、增殖等过程。肿瘤细胞异常表达CD70可促进肿瘤进展。目前围绕CD70开展了多种治疗研究，如靶向抗体，旨在阻断其促癌作用。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础科研中探索CD70在免疫调控、肿瘤发生发展中的作用，或用于药物研发中评估CD70表达水平变化本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD 27L ELISA Kit; CD 70 ELISA Kit; CD27 L ELISA Kit; CD27 LG ELISA Kit; CD27 ligand ELISA Kit; CD27-L ELISA Kit; CD27L ELISA Kit; CD27LG ELISA Kit; CD70 ELISA Kit; CD70 antigen ELISA Kit; CD70 molecule ELISA Kit; CD70_HUMAN ELISA Kit; Ki 24 antigen ELISA Kit; Ki24 antigen ELISA Kit; Surface antigen CD70 ELISA Kit; TNFSF 7 ELISA Kit; TNFSF7 ELISA Kit; Tumor necrosis factor (ligand) superfamily, member 7 ELISA Kit; Tumor necrosis factor ligand superfamily member 7 ELISA Kit
缩写：CD70
Uniprot No.：P32970
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||||||||
| Sample | Serum(n=4) | ||||||||||||
| 1:1 | Average % | 94 | |||||||||||
| Range % | 90-98 | ||||||||||||
| 1:2 | Average % | 92 | |||||||||||
| Range % | 89-97 | ||||||||||||
| 1:4 | Average % | 93 | |||||||||||
| Range % | 90-96 | ||||||||||||
| 1:8 | Average % | 96 | |||||||||||
| Range % | 90-101 | ||||||||||||
回收率：
| The recovery of human CD70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||||||||
| Sample Type | Average % Recovery | Range | |||||||||||
| Serum (n=5) | 100 | 95-105 | |||||||||||
| EDTA plasma (n=4) | 97 | 90-104 | |||||||||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||
| ng/mlOD1OD2AverageCorrected102.1962.1892.1932.08551.6991.6711.6851.5772.51.1661.1821.1741.0661.250.7660.7120.7390.6310.6250.5030.5260.5150.4070.3120.3520.3270.3400.2320.1560.2060.2160.2110.10300.1070.1090.108 | |||||||||||||
货期：3-5 working days
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