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产品描述：人CD68分子(CD68)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD68含量。CD68是一种在巨噬细胞、单核细胞等免疫细胞中表达的跨膜糖蛋白，参与吞噬活动、溶酶体代谢及与细胞和病原体的相互作用。CD68作为巨噬细胞标志物，在肿瘤免疫微环境中发挥重要作用，其表达水平与肿瘤的发生发展、预后密切相关。研究CD68的机制有助于开发针对肿瘤的新疗法。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，CD68作为巨噬细胞和单核细胞表面特异性表达的溶酶体相关膜蛋白，是先天免疫研究和炎症反应机制探索的重要标志物，广泛用于动脉粥样硬化、肿瘤微环境、组织修复等病理生理过程的机制研究。实验方案兼容常规实验室操作流程，可高效检测巨噬细胞浸润程度或免疫细胞激活状态，为免疫相关疾病模型构建、药物干预效果评估提供定量数据支持。本产品特别适用于体外研究巨噬细胞介导的免疫调控机制，或通过组织样本分析病理状态下CD68的表达水平变化，为炎症性疾病、肿瘤免疫治疗等基础研究领域提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD68; Macrosialin; Gp110; CD antigen CD68
缩写：CD68
Uniprot No.：P34810
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD68 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 90-104 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human CD68 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 92 | 85-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10001.8591.8931.8761.6655001.5561.5671.5621.3512501.1791.1981.1890.9781250.8800.9510.9160.70562.50.6130.6230.6180.40731.20.3900.4120.4010.19015.60.2930.3040.2990.08800.2100.2110.211 | ||||||
货期：3-5 working days
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