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Human decay-accelerating factor(DAF/CD55)ELISA Kit

  • 中文名： 人衰变加速因子(DAF/CD55)ELISA Kit
货号: IPD-E05121h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人衰变加速因子(DAF/CD55)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD55含量。CD55是一种重要靶点。它是补体激活调节剂，在维持机体补体平衡中发挥关键作用。相关研究围绕其在肿瘤、自身免疫病等疾病中的异常表达及功能展开，旨在通过干预CD55调节补体活性，为疾病治疗提供新策略。试剂盒检测范围为0.39 ng/ml- 25ng/ml，灵敏度为0.09 ng/ml。该产品可应用于基础科研领域，例如探究补体系统相关疾病模型中DAF/CD55的蛋白表达动态变化，评估炎症微环境中补体调控机制，或用于药物开发过程中靶向补体通路的生物标志物分析，为研究DAF/CD55在免疫调节、细胞信号传导及病理过程中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    CD 55 ELISA Kit; CD55 ELISA Kit; CD55 antigen ELISA Kit; CD55 Cromer blood group system ELISA Kit; CD55 molecule (Cromer blood group) ELISA Kit; CD55 molecule ELISA Kit; CD55 molecule; decay accelerating factor for complement (Cromer blood group) ELISA Kit; Cd55a ELISA Kit; Complement decay accelerating factor ELISA Kit; Complement decay-accelerating factor ELISA Kit; Complement decay-accelerating factor; GPI-anchored ELISA Kit; CR ELISA Kit; CROM ELISA Kit; Cromer Blood Group antigen ELISA Kit; Cromer blood group system ELISA Kit; DAF ELISA Kit; Daf-GPI ELISA Kit; DAF_HUMAN ELISA Kit; Daf1 ELISA Kit; Dcay accelerating factor for complement (CD55; Cromer blood group system) ELISA Kit; Decay accelarating factor 1; isoform CRA_a ELISA Kit; Decay accelerating factor (GPI-form) ELISA Kit; Decay Accelerating Factor for Complement ELISA Kit; Decay accelerating factor GPI-form ELISA Kit; Decay accelerating factor soluble-form ELISA Kit; GPI-DAF ELISA Kit; TC ELISA Kit
  • 缩写：
    CD55
  • Uniprot No.：
    P08174
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.39 ng/ml- 25ng/ml
  • 灵敏度：
    0.09 ng/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human DAF/CD55 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    87


    Range %

    83-91


    1:2

    Average %

    105


    Range %

    101-109


    1:4

    Average %

    94


    Range %

    88-98


    1:8

    Average %

    85


    Range %

    80-91


  • 回收率：

    The recovery of human DAF/CD55 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    93

    88-97


    EDTA plasma (n=4)

    106

    102-109


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    25

    2.465

    2.398

    2.432

    2.258


    12.5

    1.943

    1.803

    1.873

    1.699


    6.25

    1.198

    1.171

    1.185

    1.011


    3.12

    0.742

    0.752

    0.747

    0.573


    1.56

    0.441

    0.439

    0.440

    0.266


    0.78

    0.316

    0.307

    0.312

    0.138


    0.39

    0.252

    0.247

    0.250

    0.076


    0

    0.175

    0.173

    0.174




  • 货期：
    3-5 working days

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