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产品描述：人CAMPATH-1抗原(CD52)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CD52含量。CD52是位于淋巴细胞表面的糖蛋白。其作为靶点，在免疫相关疾病治疗研究中受关注。通过开发针对CD52的药物，与该靶点结合，清除表达CD52的淋巴细胞，以调节机体免疫反应，用于如多发性硬化症等疾病治疗。试剂盒检测范围为78 ng/mL-5000 ng/mL，本试剂盒适用于科研场景中CD52蛋白表达的定量分析，如免疫细胞功能研究、疾病模型中的免疫机制探索或抗体药物开发中靶标水平的监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cambridge pathology 1 antigen ELISA Kit; CAMPATH 1 ELISA Kit; CAMPATH 1 antigen ELISA Kit; CAMPATH-1 antigen ELISA Kit; CD 52 ELISA Kit; CD52 ELISA Kit; CD52 antigen ELISA Kit; CD52 molecule ELISA Kit; CD52_HUMAN ELISA Kit; CDw52 ELISA Kit; CDW52 antigen ELISA Kit; Epididymal secretory protein E5 ELISA Kit; Epididymis secretory sperm binding protein Li 171mP ELISA Kit; HE 5 ELISA Kit; He5 ELISA Kit; Human epididymis-specific protein 5 ELISA Kit
缩写：CD52
Uniprot No.：P31358
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：78 ng/mL-5000 ng/mL
灵敏度：19.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD52 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 89-102 | |||||
回收率：
| The recovery of human CD52 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 80-100 | ||||
| EDTA plasma (n=4) | 98 | 91-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected50001.9751.9521.9641.87025001.6121.5861.5991.50512501.2721.1711.2221.1286250.9840.9320.9580.8643120.6650.6290.6470.5531560.3980.3680.3830.289780.1930.2010.1970.10300.0970.0910.094 | ||||||
货期：3-5 working days
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