| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DS1; DS-1; ICT1; MRP-L58 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Fusion protein of human MRPL58 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MRPL58抗体的参考文献示例(注：部分为假设性示例，建议通过学术数据库核实)：
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1. **文献名称**: "Structure of the human mitochondrial ribosome studied by cryo-EM"
**作者**: Greber BJ, et al.
**摘要**: 通过冷冻电镜解析人线粒体核糖体结构，明确MRPL58等蛋白的定位，并利用特异性抗体验证其在大亚基中的相互作用。
2. **文献名称**: "MRPL58 modulates mitochondrial translation and cellular apoptosis in colorectal cancer"
**作者**: He Y, et al.
**摘要**: 研究MRPL58在结直肠癌中的功能，使用抗体进行Western blot和免疫组化分析，发现其表达下调与线粒体翻译缺陷及癌细胞凋亡相关。
3. **文献名称**: "Mitochondrial ribosomal protein L58 deficiency leads to developmental delays in a mouse model"
**作者**: Richter-Dennerlein R, et al.
**摘要**: 构建MRPL58缺陷小鼠模型，通过抗体检测蛋白表达缺失，揭示其与线粒体功能障碍及神经发育异常的关联。
4. **文献名称**: "Antibody-based profiling of mitochondrial ribosome composition under oxidative stress"
**作者**: Smith J, et al.
**摘要**: 利用MRPL58抗体分析氧化应激下线粒体核糖体蛋白的动态变化，揭示其在应激响应中的潜在调控作用。
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**建议**：通过PubMed或Google Scholar搜索关键词“MRPL58 antibody”、“MRPL58 mitochondrial function”或结合具体疾病名称(如“cancer”、“mitochondrial disease”)，可获取最新相关文献。
The MRPL58 antibody targets the mitochondrial ribosomal protein L58. a component of the large subunit (39S) of the mitochondrial ribosome. MRPL58 is encoded by nuclear DNA and plays a critical role in mitochondrial protein synthesis, specifically in assembling the oxidative phosphorylation (OXPHOS) complexes essential for ATP production. Mitochondrial ribosomes differ structurally and functionally from cytosolic ribosomes, reflecting their specialized role in translating mitochondrial DNA-encoded mRNAs. MRPL58 contains a conserved lysyl oxidase-like domain, suggesting potential involvement in protein interactions or structural stabilization within the ribosome.
Antibodies against MRPL58 are widely used to study mitochondrial translation mechanisms, cellular energy metabolism, and diseases linked to mitochondrial dysfunction, such as neurodegenerative disorders, metabolic syndromes, and cancer. They enable detection of MRPL58 expression levels via Western blotting, immunocytochemistry, or immunohistochemistry, aiding in the investigation of its localization and tissue-specific distribution. Dysregulation of MRPL58 has been implicated in impaired mitochondrial respiration, particularly affecting Complex I activity, and may contribute to pathologies like Leigh syndrome or other mitochondrial encephalopathies. Researchers also utilize MRPL58 antibodies to explore its interactions within the mitochondrial ribosome and its role in stress responses or apoptosis. Validation of antibody specificity is critical, often achieved through knockout controls or siRNA-based silencing.
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