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产品描述：人白细胞表面抗原CD47(CD47)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的CD47含量。CD47是一种广泛表达于多种细胞表面的跨膜蛋白。它作为“别吃我”信号分子，可与巨噬细胞表面SIRPα结合抑制吞噬作用。肿瘤细胞高表达CD47逃避免疫清除。当前多通过阻断CD47-SIRPα信号轴开展免疫治疗研究。试剂盒检测范围为3.12 pg/mL-200 pg/mL，该产品可应用于基础科研领域，例如探究肿瘤微环境中CD47的表达调控机制、评估炎症性疾病模型中的免疫信号通路变化，或用于体外药物筛选实验中靶向CD47/SIRPα通路抑制剂的功效验证，为免疫治疗研究提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Antigen identified by monoclonal ELISA Kit 1D8 ELISA Kit; Antigenic surface determinant protein OA3 ELISA Kit; CD 47 ELISA Kit; CD47 ELISA Kit; CD47 antigen (Rh-related antigen; integrin-associated signal transducer) ELISA Kit; CD47 antigen ELISA Kit; CD47 glycoprotein ELISA Kit; CD47 molecule ELISA Kit; CD47_HUMAN ELISA Kit; IAP ELISA Kit; Integrin Associated Protein ELISA Kit; Integrin associated signal transducer ELISA Kit; Integrin-associated protein ELISA Kit; Leukocyte surface antigen CD47 ELISA Kit; MER 6 ELISA Kit; MER6 ELISA Kit; OA 3 ELISA Kit; OA3 ELISA Kit; OTTHUMP00000041152 ELISA Kit; OTTHUMP00000041153 ELISA Kit; Protein MER6 ELISA Kit; Rh related antigen ELISA Kit; Surface antigen identified by monoclonal ELISA Kit 1D8 ELISA Kit
缩写：CD47
Uniprot No.：Q08722
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD47 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 96 | |||||
| Range % | 90-101 | ||||||
| 1:2 | Average % | 106 | |||||
| Range % | 102-109 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 90-98 | ||||||
| 1:8 | Average % | 88 | |||||
| Range % | 85-94 | ||||||
回收率：
| The recovery of human CD47 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 103 | 99-106 | |||||
| EDTA plasma (n=4) | 93 | 91-95 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 2001.9411.9251.9331.802 1001.6401.5191.5801.449 501.1221.1321.1270.996 250.6740.6240.6490.518 12.50.4410.4500.4460.315 6.250.3010.3270.3140.183 3.120.2030.1940.1990.068 00.1330.1280.131 | |||||||
货期：3-5 working days
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