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产品描述：人CD44分子(CD44)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CD44含量。CD44是一种跨膜糖蛋白，广泛分布于多种细胞表面。它与肿瘤的发生、发展、转移及预后密切相关。其研究机制主要是通过与透明质酸等配体结合，参与细胞黏附、迁移和信号传导等过程，是肿瘤治疗领域有潜力的靶点。试剂盒检测范围为78 pg/mL-5000 pg/mL，该产品适用于科研领域研究CD44在肿瘤侵袭转移机制、免疫细胞活化调控、慢性炎症微环境中的作用，也可用于评估药物干预或基因编辑对CD44表达水平的影响，为细胞信号通路研究及疾病模型构建提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：LHR ELISA Kit; BA-1 ELISA Kit; CD 44 ELISA Kit; CD44 ELISA Kit; CD44 antigen ELISA Kit; CD44 molecule (Indian blood group) ELISA Kit; CD44 molecule ELISA Kit; CD44_HUMAN ELISA Kit; CDw44 ELISA Kit; CDW44 antigen ELISA Kit; Cell surface glycoprotein CD44 ELISA Kit; chondroitin sulfate proteoglycan 8 ELISA Kit; CSPG8 ELISA Kit; ECMR-III ELISA Kit; Epican ELISA Kit; Extracellular matrix receptor III ELISA Kit; GP90 lymphocyte homing/adhesion receptor ELISA Kit; HCELL ELISA Kit; hematopoietic cell E- and L-selectin ligand ELISA Kit; Heparan sulfate proteoglycan ELISA Kit; Hermes antigen ELISA Kit; homing function and Indian blood group system ELISA Kit; HSA ELISA Kit; HUTCH-I ELISA Kit; HUTCH1 ELISA Kit; HUTCHI ELISA Kit; Hyaluronate receptor ELISA Kit; IN ELISA Kit; INLU-related p80 Glycoprotein ELISA Kit; MC56 ELISA Kit; MDU2 ELISA Kit; MDU3 ELISA Kit; MGC10468 ELISA Kit; MIC4 ELISA Kit; MUTCH I ELISA Kit; MUTCH1 ELISA Kit; PGP-1 ELISA Kit; PGP-I ELISA Kit; PGP1 ELISA Kit; Phagocytic glycoprotein 1 ELISA Kit; Phagocytic glycoprotein I ELISA Kit; Soluble CD44 ELISA Kit
缩写：CD44
Uniprot No.：P16070
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD44 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 88 | ||||
| Range % | 80-92 | |||||
| 1:200 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:400 | Average % | 104 | ||||
| Range % | 92-110 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human CD44 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected50002.7492.7332.7412.62925001.8231.8011.8121.70012501.0721.0441.0580.9466250.5990.5620.5810.4693120.3750.3540.3650.2531560.2410.2350.2380.126780.1810.1690.1750.06300.1160.1080.112 | ||||||
货期：3-5 working days
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