首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人膜辅蛋白(MCP/CD46)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD46含量。CD46是细胞膜上的补体调节蛋白，可保护自身组织细胞免受补体介导的免疫攻击。在研究机制方面，它能参与多种细胞过程，如病毒感染、免疫调节等，也是某些病毒进入宿主细胞的受体，目前围绕它在疾病治疗尤其是抗肿瘤治疗上有诸多探索。试剂盒检测范围为3.12 ng/mL-200 ng/mL，本试剂盒适用于基础医学研究中探索疾病模型中MCP/CD46的蛋白表达动态，如通过检测炎症性疾病患者血浆样本或肿瘤组织匀浆中的蛋白浓度变化，揭示其在补体激活通路中的调控机制，为免疫学、肿瘤学及感染性疾病研究提供可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD46; MCP; MIC10; Membrane cofactor protein; TLX; Trophoblast leukocyte common antigen; CD antigen CD46
缩写：CD46
Uniprot No.：P15529
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 93-102 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 87-94 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 85-93 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 90-95 | |||||
回收率：
| The recovery of human MCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-99 | ||||
| EDTA plasma (n=4) | 99 | 94-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.1432.2532.1982.0271001.5441.5631.5541.383501.0771.1701.1240.953250.7740.7960.7850.61412.50.5130.5260.5200.3496.250.3680.3910.3800.2093.120.2730.2930.2830.11200.1700.1720.171 | ||||||
货期：3-5 working days
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