首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠可溶性CD40配体(sCD40L)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD40LG含量。CD40LG，全称CD40配体，是一种肿瘤坏死因子超家族成员，主要表达于T细胞表面，通过与抗原呈递细胞上的CD40结合，参与免疫反应和肿瘤生长调控。研究显示，CD40LG在乳腺癌等肿瘤中表达异常，可能成为预后预测和治疗的新靶点。试剂盒检测范围为1.87 ng/mL-120 ng/mL，本试剂盒适用于研究心血管疾病、慢性炎症或免疫相关疾病模型中 sCD40L 的动态变化，可兼容多种生物样本类型，为探索其生物学功能及病理机制提供可靠工具。满足科研场景下对大鼠 sCD40L 的精准定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cd40lg ELISA Kit; Cd40l ELISA Kit; Tnfsf5CD40 ligand ELISA Kit; CD40-L ELISA Kit; Tumor necrosis factor ligand superfamily member 5 ELISA Kit; CD antigen CD154) [Cleaved into: CD40 ligand ELISA Kit; membrane form; CD40 ligand ELISA Kit; soluble form ELISA Kit; sCD40L)] ELISA Kit
缩写：CD40LG
Uniprot No.：Q9Z2V2
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：1.87 ng/mL-120 ng/mL
灵敏度：0.47 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat sCD40L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 83-98 | |||||
| 1:2 | Average % | 93 | ||||
| Range % | 87-98 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 83-95 | |||||
| 1:8 | Average % | 103 | ||||
| Range % | 95-107 | |||||
回收率：
| The recovery of rat sCD40L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 82-92 | ||||
| EDTA plasma (n=4) | 95 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1202.4872.6882.5882.437601.7341.6431.6891.538301.0491.1061.0780.927150.6740.6850.6800.5297.50.4420.4570.4500.2993.750.3140.3270.3210.1701.870.2360.2450.2410.09000.1500.1510.151 | ||||||
货期：3-5 working days
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