首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠CD4分子(CD4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD4含量。CD4是人体免疫系统的关键靶点。它是T细胞表面糖蛋白，辅助免疫细胞识别外来抗原。在研究机制上，CD4可与抗原呈递细胞结合，激活T细胞，启动免疫应答，在抗感染、抗肿瘤等方面作用重大，也是艾滋病病毒主要攻击对象。试剂盒检测范围为0.78 U/mL-50 U/mL，适用于免疫学基础研究、自身免疫性疾病模型评估、感染性疾病机制探索等方向，可为实验动物模型中T细胞功能分析、免疫调节药物开发等研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cd4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; W3/25 antigen; CD antigen CD4
缩写：CD4
Uniprot No.：P05540
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 U/mL-50 U/mL
灵敏度：0.195 U/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 87-102 | ||||||
| 1:2 | Average % | 101 | |||||
| Range % | 94-105 | ||||||
| 1:4 | Average % | 99 | |||||
| Range % | 88-103 | ||||||
| 1:8 | Average % | 105 | |||||
| Range % | 98-109 | ||||||
回收率：
| The recovery of rat CD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 92 | 85-98 | |||||
| EDTA plasma (n=4) | 98 | 89-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| U/mlOD1OD2AverageCorrected 502.6562.5872.6222.508 252.1712.0622.1172.003 12.51.4381.3881.4131.299 6.250.7200.7150.7180.604 3.120.4120.4240.4180.304 1.560.2610.2520.2570.143 0.780.1760.1830.1800.066 00.1120.1150.114 | |||||||
货期：3-5 working days
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