| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ARL2BP |
| Uniprot No | Q9Y2Y0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-163aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMDALEGESFALSFSSASDAEFDAVVGYLED IIMDDEFQLLQRNFMDKYYLEFEDTEENKLIYTPIFNEYISLVEKYIEEQ LLQRIPEFNMAAFTTTLQHHKDEVAGDIFDMLLTFTDFLAFKEMFLDYRA EKEGRGLDLSSGLVVTSLCKSSSLPASQNNLRH |
| 预测分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ARL2BP重组蛋白的3篇代表性文献摘要概括：
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1. **文献名称**：*Structural and functional characterization of recombinant ARL2BP in cilia assembly*
**作者**：Smith J, et al.
**摘要**：研究利用大肠杆菌系统成功表达并纯化ARL2BP重组蛋白，通过X射线晶体学解析其三维结构，发现其与BART结构域的相互作用，并证实其在纤毛形成中调控ARL2-GTP酶活性的关键作用。
2. **文献名称**：*ARL2BP interacts with PDE6D to regulate photoreceptor cell viability*
**作者**：Li X, et al.
**摘要**：通过哺乳动物细胞表达重组ARL2BP，结合免疫共沉淀技术，揭示其与磷酸二酯酶PDE6D的相互作用，并证明该复合物参与视网膜感光细胞中脂质修饰蛋白的运输及细胞存活调控。
3. **文献名称**：*Proteomic analysis of ARL2BP-associated pathways in cancer cells*
**作者**：Wang Y, et al.
**摘要**：利用HEK293细胞表达His标签重组ARL2BP，通过质谱筛选其结合蛋白网络，发现其与线粒体代谢酶及微管动力学的关联，提示ARL2BP可能通过调控细胞能量代谢影响肿瘤转移。
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注：以上文献为示例性概括，实际文献需通过PubMed/Google Scholar以关键词“ARL2BP recombinant”或“ARL2BP structure/function”检索。
**Background of ARL2BP Recombinant Protein**
ARL2BP (ADP-ribosylation factor-like 2 binding protein), also known as BART (Binder of ARL2), is a conserved eukaryotic protein that plays critical roles in cellular processes, including cytoskeletal organization, intracellular trafficking, and ciliogenesis. It functions primarily by interacting with ARL2 (ADP-ribosylation factor-like 2), a small GTPase involved in regulating microtubule dynamics and mitochondrial function. The ARL2BP-ARL2 complex is essential for maintaining structural integrity in cells, particularly in high-energy-demanding tissues like the retina and neuronal systems.
Structurally, ARL2BP contains a central α-helical domain that facilitates its binding to ARL2 in a GTP-dependent manner. This interaction stabilizes ARL2 in its active GTP-bound state, enabling downstream signaling. Studies also link ARL2BP to the assembly and maintenance of primary cilia, sensory organelles critical for signal transduction in development and homeostasis. Dysregulation of ARL2BP has been implicated in ciliopathies, retinal degeneration, and developmental disorders.
Recombinant ARL2BP proteins are engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, biologically active forms for research. These proteins retain the ability to bind ARL2 and are utilized in *in vitro* assays, structural studies, and functional analyses to dissect molecular mechanisms underlying ARL2BP-associated pathways. Tagged versions (e.g., His-, GST-, or FLAG-tagged) enable easy purification and detection.
Research on ARL2BP recombinant proteins has advanced understanding of its role in diseases and potential therapeutic targets. For instance, mutations in ARL2BP are linked to autosomal recessive retinal degeneration, highlighting its importance in photoreceptor survival. Additionally, its involvement in ciliary defects underscores its relevance in disorders like Bardet-Biedl syndrome. Overall, ARL2BP recombinant proteins serve as vital tools for exploring cell biology, disease mechanisms, and drug discovery.
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