首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠CC趋化因子受体2(CCR2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CCR2含量。CCR2作为一种趋化因子受体，在人体免疫调节等过程中发挥重要作用。其研究机制主要聚焦于它与趋化因子的相互作用，介导白细胞迁移。通过调控CCR2活性，有望干预炎症反应、肿瘤转移等病理过程，为相关疾病治疗提供新思路。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研领域对大鼠模型中CCR2表达水平的动态监测，如炎症相关疾病机制研究、代谢综合征模型评估或药物干预效果分析；为探索CCR2在免疫调节、细胞迁移及疾病发展中的功能提供可靠工具，特别适用于动物实验中的生物标志物定量及信号通路研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ccr2; Cmkbr2; C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CCR-2; CCR2; CD antigen CD192
缩写：CCR2
Uniprot No.：O55193
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CCR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 102 | ||||
| Range % | 96-108 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 98 | ||||
| Range % | 92-102 | |||||
回收率：
| The recovery of rat CCR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-99 | ||||
| EDTA plasma (n=4) | 92 | 88-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.9162.8152.8662.7455002.2322.1312.1822.0612501.4611.4401.4511.3301250.7930.8020.7980.67762.50.4480.4370.4430.32231.250.2530.2420.2480.12715.60.1720.1690.1710.05000.1200.1220.121 | ||||||
货期：3-5 working days
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